Exoenzymes,. protease (P) and elastase (E) produced by Pseudomonas aeruginosa are reported to have close relationship with pathogen city of Pseudomonas aeruginosa. Productibility of exoenzymes P and E were studied and compared in environmental isolates from hospital environments and clinical isolates. from various clinical specimens, also, the relationship between. their enzyme production and serotypes were reviewed.
1. Clinical isolates were typed into nine serotypes A, B, C, D, E, F, G, H and I. Serotype E had the highest incidence of 24%, followed by B with 16.8%, G, 15. 1% and C, 9.3%.
2. Environmental isolates were typed as serotype B, C, E, F, G, H, I, K and M. Serotype I had the highest incidence of 26.6%, followed by C, F and M each incidence of 14.3%.
3. In the typing of the above two groups, serotypes A and D were found only in the clinical isolates and serotypes K and M were fund only in the environmental isolates. Serotypes J and L were found in neither clinical isolates nor environmental isolates.
4. In the distribution of serotypes from various clinical specimens, serotype G among isolates from pus showed incidence of 20.4%, and serotypes E and B were 19.5 % separately. Serotype E had incidence of 22.6% and 20.0% in urine and sputa respectively, showing a high rate compared to the other serotypes.
5. The incidence of strains producing both exoenzymes P and E was 77.8% in the preserved strains of clinical isolates and 76. 2% in the environmental isolates. There were no significant difference between the two groups.
6. Serotypes A and H, which are preserved strains from clinical isolates showed productibility of both
enzymes P and E, the other serotypes showed productibility of various combination of exoenzymes_ Among the environmental isolates, production of both exoenzymes P and E were seen in serotypes E, F, G; H, I and K and no serotype produced only P or E.
In ability to produce exoenzymes of isolates from sources of various clinical specimens, strains producing both exoenzymes P and E were found most frequently in pus with incidence rate of 82. 0%, followed by 80.0% in sputum and urine.
8. Almost all the fresh strains of clinical isolates were producers of both exoenzymes P and E.
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